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bacterial species k pneumoniae  (DSMZ)


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    DSMZ bacterial species k pneumoniae
    Cytokine production of PBMCs incubated with K. <t>pneumoniae</t> previously exposed to Mars-like conditions. PBMC innate ( A ) and adaptive ( B ) cytokine responses. n = 9 biological replicates pooled from three independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. M9, minimal media; Unst, unstimulated; UV, ultraviolet.
    Bacterial Species K Pneumoniae, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+species+k+pneumoniae/Brevibacillus+borstelensis/pmc12421960-121-1-17
    Average 93 stars, based on 6 article reviews
    bacterial species k pneumoniae - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions"

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

    Journal: mBio

    doi: 10.1128/mbio.01099-25

    Cytokine production of PBMCs incubated with K. pneumoniae previously exposed to Mars-like conditions. PBMC innate ( A ) and adaptive ( B ) cytokine responses. n = 9 biological replicates pooled from three independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. M9, minimal media; Unst, unstimulated; UV, ultraviolet.
    Figure Legend Snippet: Cytokine production of PBMCs incubated with K. pneumoniae previously exposed to Mars-like conditions. PBMC innate ( A ) and adaptive ( B ) cytokine responses. n = 9 biological replicates pooled from three independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. M9, minimal media; Unst, unstimulated; UV, ultraviolet.

    Techniques Used: Incubation, Comparison

    Effector functions of PBMCs against bacteria exposed to Mars-like conditions. Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae ( A ) or S. marcescens ( B ). Percentage of CD11b+ cells positive for DTAF (FITC)-labeled bacteria ( C and E ) and representative histograms ( D and F ) for K. pneumoniae and S. marcescens . n = 6–9 biological replicates from two to three independent experiments, * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 Friedman test followed by Dunn’s multiple comparison test. AUC, area under curve as a ratio of ROS production over time; M9, minimal media; Unst, unstimulated; UV, ultraviolet.
    Figure Legend Snippet: Effector functions of PBMCs against bacteria exposed to Mars-like conditions. Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae ( A ) or S. marcescens ( B ). Percentage of CD11b+ cells positive for DTAF (FITC)-labeled bacteria ( C and E ) and representative histograms ( D and F ) for K. pneumoniae and S. marcescens . n = 6–9 biological replicates from two to three independent experiments, * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 Friedman test followed by Dunn’s multiple comparison test. AUC, area under curve as a ratio of ROS production over time; M9, minimal media; Unst, unstimulated; UV, ultraviolet.

    Techniques Used: Bacteria, Labeling, Comparison

    Regrowth after desiccation partially rescues the effects of desiccation. Cytokine production following stimulation of PBMCs for 24 h ( A and E ) and 7 days ( B and F ) with K. pneumoniae ( A and B ) or S. marcescens ( E and F ). Reactive oxygen species production when stimulated with K. pneumoniae ( C ) or S. marcescens ( G ). Phagocytosis of K. pneumoniae ( D ) and S. marcescens ( H ) by CD11b+ cells. n = 6 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test with Dunn’s multiple comparison test. M9, minimal media.
    Figure Legend Snippet: Regrowth after desiccation partially rescues the effects of desiccation. Cytokine production following stimulation of PBMCs for 24 h ( A and E ) and 7 days ( B and F ) with K. pneumoniae ( A and B ) or S. marcescens ( E and F ). Reactive oxygen species production when stimulated with K. pneumoniae ( C ) or S. marcescens ( G ). Phagocytosis of K. pneumoniae ( D ) and S. marcescens ( H ) by CD11b+ cells. n = 6 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test with Dunn’s multiple comparison test. M9, minimal media.

    Techniques Used: Comparison

    The morphology of bacterial cells is altered following exposure to simulated Martian conditions. TEM visualization of K. pneumoniae ( A ) and S. marcescens ( C ), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae ( B ) and S. marcescens ( D ). Scale bars are 500 nm in panel A and 250 nm in panel C. n = 5–7 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. FSC, forward scatter; M9, minimal media; SSC, side scatter; UV, ultraviolet.
    Figure Legend Snippet: The morphology of bacterial cells is altered following exposure to simulated Martian conditions. TEM visualization of K. pneumoniae ( A ) and S. marcescens ( C ), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae ( B ) and S. marcescens ( D ). Scale bars are 500 nm in panel A and 250 nm in panel C. n = 5–7 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. FSC, forward scatter; M9, minimal media; SSC, side scatter; UV, ultraviolet.

    Techniques Used: Flow Cytometry, Comparison

    Related Articles

    Incubation:

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 , and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by Domínguez-Andrés et al. ( ).Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Article Title: Immune responses to human pathogens exposed to simulated Mars conditions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by .Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Comparison:

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 , and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by Domínguez-Andrés et al. ( ).Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Article Title: Immune responses to human pathogens exposed to simulated Mars conditions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by .Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Bacteria:

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 , and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by Domínguez-Andrés et al. ( ).Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Article Title: Immune responses to human pathogens exposed to simulated Mars conditions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by .Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Labeling:

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 , and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by Domínguez-Andrés et al. ( ).Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Article Title: Immune responses to human pathogens exposed to simulated Mars conditions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by .Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Flow Cytometry:

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 , and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by Domínguez-Andrés et al. ( ).Following growth in nutrient broth (NB, Difco) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..

    Article Title: Immune responses to human pathogens exposed to simulated Mars conditions
    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.. Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4 , 0.1 mM CaCl 2 and 0.02 mM Fe(III)Cl 3 without carbon source) as described previously by .Following growth in Nutrient Broth (Difco; NB) and on NB agar (15 g/L), at 35°C overnight, colonies from each species were incubated in M9-complete minimal salts media (47.8 mM Na 2 HPO 4 , 22.0 mM KH 2 PO 4 , 8.6 mM NaCl, 3.7 mM NH 4 Cl, 2 mM MgSO 4..



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    Cytokine production of PBMCs incubated with K. pneumoniae previously exposed to Mars-like conditions. PBMC innate ( A ) and adaptive ( B ) cytokine responses. n = 9 biological replicates pooled from three independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. M9, minimal media; Unst, unstimulated; UV, ultraviolet.

    Journal: mBio

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

    doi: 10.1128/mbio.01099-25

    Figure Lengend Snippet: Cytokine production of PBMCs incubated with K. pneumoniae previously exposed to Mars-like conditions. PBMC innate ( A ) and adaptive ( B ) cytokine responses. n = 9 biological replicates pooled from three independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. M9, minimal media; Unst, unstimulated; UV, ultraviolet.

    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

    Techniques: Incubation, Comparison

    Effector functions of PBMCs against bacteria exposed to Mars-like conditions. Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae ( A ) or S. marcescens ( B ). Percentage of CD11b+ cells positive for DTAF (FITC)-labeled bacteria ( C and E ) and representative histograms ( D and F ) for K. pneumoniae and S. marcescens . n = 6–9 biological replicates from two to three independent experiments, * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 Friedman test followed by Dunn’s multiple comparison test. AUC, area under curve as a ratio of ROS production over time; M9, minimal media; Unst, unstimulated; UV, ultraviolet.

    Journal: mBio

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

    doi: 10.1128/mbio.01099-25

    Figure Lengend Snippet: Effector functions of PBMCs against bacteria exposed to Mars-like conditions. Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae ( A ) or S. marcescens ( B ). Percentage of CD11b+ cells positive for DTAF (FITC)-labeled bacteria ( C and E ) and representative histograms ( D and F ) for K. pneumoniae and S. marcescens . n = 6–9 biological replicates from two to three independent experiments, * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 Friedman test followed by Dunn’s multiple comparison test. AUC, area under curve as a ratio of ROS production over time; M9, minimal media; Unst, unstimulated; UV, ultraviolet.

    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

    Techniques: Bacteria, Labeling, Comparison

    Regrowth after desiccation partially rescues the effects of desiccation. Cytokine production following stimulation of PBMCs for 24 h ( A and E ) and 7 days ( B and F ) with K. pneumoniae ( A and B ) or S. marcescens ( E and F ). Reactive oxygen species production when stimulated with K. pneumoniae ( C ) or S. marcescens ( G ). Phagocytosis of K. pneumoniae ( D ) and S. marcescens ( H ) by CD11b+ cells. n = 6 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test with Dunn’s multiple comparison test. M9, minimal media.

    Journal: mBio

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

    doi: 10.1128/mbio.01099-25

    Figure Lengend Snippet: Regrowth after desiccation partially rescues the effects of desiccation. Cytokine production following stimulation of PBMCs for 24 h ( A and E ) and 7 days ( B and F ) with K. pneumoniae ( A and B ) or S. marcescens ( E and F ). Reactive oxygen species production when stimulated with K. pneumoniae ( C ) or S. marcescens ( G ). Phagocytosis of K. pneumoniae ( D ) and S. marcescens ( H ) by CD11b+ cells. n = 6 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test with Dunn’s multiple comparison test. M9, minimal media.

    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

    Techniques: Comparison

    The morphology of bacterial cells is altered following exposure to simulated Martian conditions. TEM visualization of K. pneumoniae ( A ) and S. marcescens ( C ), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae ( B ) and S. marcescens ( D ). Scale bars are 500 nm in panel A and 250 nm in panel C. n = 5–7 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. FSC, forward scatter; M9, minimal media; SSC, side scatter; UV, ultraviolet.

    Journal: mBio

    Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

    doi: 10.1128/mbio.01099-25

    Figure Lengend Snippet: The morphology of bacterial cells is altered following exposure to simulated Martian conditions. TEM visualization of K. pneumoniae ( A ) and S. marcescens ( C ), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae ( B ) and S. marcescens ( D ). Scale bars are 500 nm in panel A and 250 nm in panel C. n = 5–7 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. FSC, forward scatter; M9, minimal media; SSC, side scatter; UV, ultraviolet.

    Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

    Techniques: Flow Cytometry, Comparison